Agnihotri's career stretches back to 1983 when he made a brief appearance as a child artist in Pasand Apni Apni. He started his film career as an adult in 1993 with Mahesh Bhatt's Sir. He went on to act in several other films throughout the 1990s and early 2000s with his most notable films being Krantiveer (1994),[3] Aatish (1994)[4] and Hum Tumhare Hain Sanam (2002).He made his directorial debut with the 2004 film Dil Ne Jise Apna Kahaa which starred his brother-in-law Salman Khan, Bhoomika Chawla, and Preity Zinta in lead roles.[3] In 2007 he started work on his second directorial film Hello which starred his brother-in-law Sohail Khan, Isha Koppikar and Sharman Joshi and was released on 10 October 2008.
His upcoming film is O Teri starring Pulkit Samrat and Sarah Jane Dias, and directed by debutant Umesh Bhisht. The production of the film is almost complete, and it is slated for a release later in the year